Safer staining method for acid fast bacilli.

Safer staining method for acid fast bacilli.

To develop a method for staining acid fast bacilli which excluded highly toxic phenol from the staining solution. A lipophilic agent, a liquid organic detergent, LOC High Studs, distributed by Amway, was substituted. The acid fast bacilli stained red; nuclei, cytoplasm, and cytoplasmic elements stained blue on a clear background. These results compare very favourably with acid fast bacilli stai...

Evaluation of a dual-staining method for acid-fast bacilli.

A dual-staining procedure for acid-fast bacilli was found to have poor correlation with the Ziehl-Neelsen and auramine-rhodamine staining techniques.

Evaluation of a Cold Staining Method for Detecting Acid Fast Bacilli in the Sputum

Tuberculosis is a disease of great importance in developing countries, such as India, where it has caused considerable morbidity and mortality. Sputum smear microscopy is the cornerstone in diagnosis of pulmonary tuberculosis, which is usually done using Ziehl–Neelsen (ZN) method (Vasanthakumari et al., 1986; Gupta et al., 2009; Chandrasekaran et al., 1991). The use of ZN Method in remote areas...

Method for staining both acid-fast and chromophobic tubercle bacilli with carbolfuschsin.

comparison of the value of two different sputum staining for diagnosis of acid fast bacilli

conclusion fm is more sensitive than zn for diagnosis of tb. however, since fm is more sensitive and rapid, using this method in clinical laboratories with large specimen numbers is recommended. results a total of 102 out of 920 study subjects had pulmonary tb, of them 68 (66.66%) patients were smear positive by either staining method while others were smear negative. the proportion of positive...